This study used a randomized controlled trial design with a mixed methods evaluation spanning from September 2023 to January 2024. A total of 1282 medical students from 9 specialties (clinical medicine, traditional Chinese medicine, pediatrics, nursing, medical imaging, preventive medicine, clinical pharmacy, acupuncture, moxibustion, and traditional Chinese pharmacy) participated in the study, with equal, random assignment to experimental (n=653) and control (n=629) groups. The teaching implementation followed a systematic schedule across 6 weeks (detailed in Figure 1), integrating theoretical lectures, virtual simulation exercises, practical laboratory sessions, and comprehensive assessments of both groups. This design enabled direct comparison of learning effectiveness between traditional and virtual simulation–enhanced teaching approaches.

The implementation of this teaching system integrated both virtual simulation and traditional laboratory approaches. The teaching process followed a systematic structure comprising preclass preparation, in-class activities, and postclass assessment. All participating students received standardized learning materials and safety protocols, with the experimental group using the virtual simulation platform under instructor guidance while the control group followed traditional laboratory teaching methods. Both groups engaged in comprehensive learning activities designed to achieve the specified learning objectives, with emphasis on laboratory safety awareness and standardized experimental procedures.

A comprehensive evaluation framework was established to assess the effectiveness of the teaching system. The assessment strategy incorporated 3 primary components: performance assessment through standardized practical tests, learning experience evaluation via structured questionnaires, and comparative cost analysis of resource use. Key evaluation indicators encompassed experimental operation proficiency, theoretical knowledge mastery, student satisfaction levels, teaching resource efficiency, and safety protocol compliance. This multidimensional assessment approach enabled thorough evaluation of both learning outcomes and teaching effectiveness.

The collected data underwent rigorous statistical analysis using SPSS (version 25.0; SPSS Inc). Statistical methods included independent 2-tailed t tests for comparing group differences in continuous variables and χ² tests for analyzing categorical data. All statistical analyses were conducted with a significance level set at P<.05. This analytical approach ensured robust evaluation of the teaching system’s effectiveness while maintaining scientific rigor in data interpretation.

This study was approved by the ethics committee of Chongqing Medical University (approval number 2023027). The study was conducted in accordance with local legislation and institutional requirements. We obtained informed consent from participants through an online questionnaire system. Participants were provided with a research information statement detailing the study’s purpose, participation requirements, potential risks and benefits, data protection measures, and a declaration that participation was voluntary. Participants were required to confirm they had read and understood this information and agreed to participate before being included in the study. No financial or material compensation was provided to participants. All data were fully anonymized prior to analysis.

The developed virtual laboratory system (Multimedia Appendix 1) successfully integrated 3 core modules: knowledge review, a learning module, and an assessment module. The experimental procedure flow (Multimedia Appendix 2) was effectively translated into the virtual environment. The 3-module design received high approval from 1214 of 1282 (94.7%) participants across all medical specialties (Multimedia Appendix 3). Figure 2 shows the CONSORT (Consolidated Standards of Reporting Trials) diagram of participation flow.

Comparative analysis revealed significant differences in test scores between the experimental and control groups (Table 1). The experimental group demonstrated higher performance across multiple medical fields, with particularly notable improvements in clinical medicine, nursing, traditional Chinese pharmacology, and the science of acupuncture and moxibustion (overall P<.001).

Student perception analysis (Table 2) highlighted key advantages of the virtual system: 90.8% of students reported enhanced safety, 79.6% appreciated the unlimited repetition capability, and 74.6% mentioned cost reduction. The standardized experimental conditions (61.6%) and personalized teaching features (50.6%) also received positive recognition.

The interface design evaluation (Multimedia Appendix 4) showed that 658 of 1282 (51.3%) students found it well designed and clear. Regarding difficulty levels (Multimedia Appendix 5), 1168 (91.1%) students found the experiment appropriately challenging, and 1156 (90.2%) students reported that the first-person perspective enhanced their learning experience (Table 3), with the 3-module design receiving high approval (Multimedia Appendix 3).

The results showed that 1268 of 1282 (98.9%) students reported improved comprehension (Table 4). Student acceptance data (Table 5) indicated that 1145 (89.3%) were willing to adopt virtual simulation as a supplementary learning tool. Overall satisfaction (Table 6) reached 97.2%, with 39.6% being “very satisfied” and 57.6% “generally satisfied.”

Virtual simulation technology offers innovative and effective solutions for teaching related to laboratory experiments [20-23]. Our program was developed to address a critical training gap identified in institutional needs assessments: over 80% of students sought enhanced training in pathogenic bacteria, yet 90% of faculty opposed live cocci experiments due to biosafety concerns.

The 3-module design (the knowledge review, learning module, and assessment module) successfully reconciled this “must-learn vs cannot-do” paradox, achieving 94.7% student approval. This structure aligns with cognitive learning theory [20] while specifically addressing the staged requirements of microbiology experiments [21]. Quantitative assessments confirmed significant improvements across specialties (P<.05), including a 98.9% enhancement in theoretical understanding and 90.8% increase in safety awareness—effectively overcoming key limitations of traditional teaching through (1) standardized experimental procedures, (2) 60% cost reduction over 5 years, and (3) integrated BSL-2 safety training.

These findings are corroborated by prior research [11,24-26], particularly regarding virtual laboratories’ advantages in safety and accessibility. The program’s cost-effectiveness and scalability (serving large student populations with easy updates) will allow the further optimization of teaching models [7-9] and expansion of remote education possibilities.

Nevertheless, real experiments retain irreplaceable advantages in cultivating practical operational skills and sensory experiences [27]. Feedback from 1.1% of students indicating limited assistance from virtual experiments highlights the persistent limitations in substituting for hands-on operations in real environments [4-6]. Consequently, virtual simulation experiments and real experiments each possess distinct advantages [13-15,28]. The optimal teaching strategy should therefore involve an organic integration of both approaches [16-18,29].

Based on tracking student feedback, we identified the main issues with the virtual experiment system as lag, lack of speed adjustment options, poor learning module integration, incomplete results analysis, and inadequate error simulation compared to real experiments. To address these problems, we developed a series of optimization strategies. First, server performance should be enhanced and the program’s code should be optimized to resolve lag issues. Second, the introduction of an intelligent time management system would allow students to adjust the experiment’s progress. In terms of instructional design, the learning modules could be improved by increasing interactive guidance and feedback. Simultaneously, incorporating data visualization tools would strengthen results analysis, and refining the simulation fidelity would allow it to better reflect real experimental conditions. Additionally, we designed a multidimensional evaluation system to continuously collect and analyze student feedback for iterative system optimization. Lastly, it is important to strengthen teacher training, which is crucial for guiding student learning through virtual experiments. Through comprehensive implementation of these strategies [30], we expect to significantly enhance the technical implementation, instructional design, and learning experience of virtual experiments, thereby substantially improving their educational effectiveness.

To ensure analytical rigor, this study used a complete case analysis method, including only data from students who completed both tests and questionnaires. While this approach enhanced data quality and analytical consistency, it also reduced the effective sample size, potentially limiting the generalizability of the results. Future research might consider advanced techniques such as multiple imputation to handle missing data and extending the scope of the study to different educational levels to enhance its representativeness. Subsequent studies should adopt a mixed methods approach, integrating quantitative analysis with qualitative research, and use a longitudinal design to assess the long-term educational effectiveness of virtual experiments. These research directions would deepen understanding of virtual experiments’ educational effectiveness, promote teaching innovation, and provide crucial guidance for future education practices.

The virtual simulation experiment system described here serves as an effective complement to real experiments, offering significant advantages in terms of safety, repeatability, and standardization. Our study demonstrates its effectiveness through improved student performance and a high satisfaction rate (97.2%). The 3-module design effectively enhanced students’ understanding (98.9% reporting improvement) and engagement with the experimental procedures. While virtual experiments show distinct advantages in visualization and allow repeated practice, they work best when integrated with traditional hands-on experiments to provide a comprehensive learning experience. This combination leverages the strengths of both approaches—the safety and flexibility of virtual simulation with the irreplaceable tactile experience of real laboratory practice. Future development should focus on technical optimization and enhanced integration with traditional teaching methods to further improve educational outcomes.